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Aquatic Plant Invasion: Simulation

Unit Plan: Invasive SpeciesTime: Three 45-minute periods over 1-2 weeks Setting: Classroom
6-8, 9-12Hudson River Ecology
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Students will know the relationship between light and dissolved oxygen and be able to predict what will happen when a plant does not receive enough light. Students will know what happens to an aquatic ecosystem when a floating macrophyte is introduced as an invasive species, and be able to design an experiment to test their hypothesis.


    1. Students discuss the impacts of living without light 2. Students learn about the impacts of light on plants using a demonstration or overnight observation; a reading is also provided 3. Students view photos of water chestnut 4. Students create an experiment to test the impact of a floating plant on dissolved oxygen 5. Over a one-week or two-week period, students take measurements of the water 6. Students report and discuss conclusions


    • jars or 2-liter bottles with the tops cut off (at least 2 jars per group)
    • aged tap water
    • fertilizer (phosphate-based dish detergent works well)
    • algae culture
    • Elodea
    • aluminum foil
    • plastic wrap
    • thermometers
    • styrofoam
    • copies of lab sheet, reading

    Teaching notes:

    Day 1: This can be done as a demonstration or as a cooperative activity. To prepare the experiment, set up several jars of aged tap water with sprigs of Elodea and place them in the dark overnight. This causes the levels of DO to drop due to respiration, and allows a better measurement of the change during the experiment. For a cooperative activity, each group needs two jars with Elodea. You should also provide jars of boiled sealed water (with no DO) that the students can test before and after to prove that the light is not affecting the water alone.

    Day 2: Once students have tested their water after leaving the jars in different light conditions, they should be presented with the water chestnut problem. Once they have read the background information, they will be given several choices for how to mimic the effects of water chestnut: using a cover on the water, such as styrofoam or aluminum foil; adding algal cultures to create a floating plant ‘mat’; or using floating plants such as duckweed (teacher’s discretion). Allow one-two weeks for the creation and completion of the experiments.

    Engage: Students should be encouraged to think about what life would be like if they had to live in a closet, or, if some imaginary large umbrella covered the sky and blocked out all the sunlight. Ask students if they know of any organisms that can survive without sunlight (they might think about animals in that live along the deep ocean vents). Discuss the kinds of adaptations these animals need to survive in such hostile conditions. Explain to students that today they will try and find out what happens to plants in different levels of light.

    Day 1: Students will be given the jars with the elodea and the control jars. They should decide how to set up their experiment. Some students may want to cover their jars with a dark cloth, while others may want to place the jar in a closet or cupboard. Either way, make sure that temperature is not a confounding variable. Students should test the DO levels before they start their experiment. Leave the jars overnight and allow the students enough time to test the water again the next day. The jars have to be kept in very low artificial light if they are left overnight, because they might produce too much oxygen and saturate the system.

    Day 2: After students test their jars, they should be shown the next set of materials: algal culture, floating plants, various types of covers for the jars, fertilizer. Each group can decide what concentration of fertilizer to use in their ‘pond’, but they should keep one jar as a control. The second jar will become inoculated with algal culture and the pre-determined amount of fertilizer. Jars should be placed in warm, well-lit place. This experiment should be allowed to run for two weeks. Replace water lost to evaporation with aged tap water. Students should continuously make observations on their ponds.

    Explain: Water chestnut is an annual plant that consists of floating leaves that are attached to the sediment by a long, tough stem. The plant produces an edible nut that is hard and spiny on the outside, and can remain viable for a decade or more. Water chestnut was introduced to North America in the late 19th century by a well-meaning botanist who thought the plant was beautiful and useful as potential wildlife food. The chestnut quickly escaped the lakes where it was introduced, becoming a nuisance in the Hudson in the 1950s. It prefers slow-moving water up to 5m deep, and once established it forms dense, impenetrable stands of roots that even a kayak can’t get through.

    There are a few problems with the water chestnut. First, because of the thick beds that it forms, it’s an obstacle for boaters and people who want to use the river recreationally. It has displaced native aquatic plants, changing the underwater habitat for fish. Because the plant’s leaves cover the water surface, blocking out sunlight, oxygen levels are reduced beneath plant beds. The roots and stems remove oxygen from the water to support respiration, while the photosynthesis that takes place happens only above the water’s surface. The water actually becomes anoxic (oxygen-free) during low tide, becoming replenished only when the tides change.

    There may also be some positive aspects related to the water chestnut, although many of these are still being investigated. It may provide a good habitat for invertebrates, some studies show higher fish diversity in the water chestnut, and it removes nutrients from the water. There have been attempts to eradicate the plant, but, it is now more abundant than it has ever been.

    Extend: Ask students to think about what their results might mean for aquatic animals living in a situation with low light. Ask the students to think about what kinds of conditions cause low light levels for submerged plants. This would be a good time to show the water chestnut photos and discuss the implications.

    Evaluate: Students should complete their lab report and be prepared to give a presentation to the class about how an invasive floating plant can affect the aquatic ecosystem.

    Lesson Files

    Lab sheet

    Benchmarks for Science Literacy

    1B Scientific Inquiry, 2A Patterns and Relationships, 4G Forces of Nature, 5D Interdependence of Life, 5E Flow of Matter and Energy

    NYS Standards

    MST 1 - Mathematical analysis, scientific inquiry, and engineering design, MST 4- Physical setting, living environment and nature of science
    Next Generation Science Standards

    Science and Engineering Practices

    Asking questions and defining problems

    Cross Cutting Concepts

    Stability and change

    Disciplinary Core Ideas

    LS2A: Interdependent Relationships in Ecosystems
    New York State Science Learning Standards

    Performance Expectations

    MS-LS2-1. Analyze and interpret data to provide evidence for the effects of resource availability on organisms and populations of organisms in an ecosystem., MS-LS2-2. Construct an explanation that predicts patterns of interactions among organisms in a variety of ecosystems., MS-LS2-4. Construct an argument supported by empirical evidence that changes to physical or biological components of an ecosystem affect populations., HS-LS2-1. Use mathematical and/or computational representations to support explanations of biotic and abiotic factors that affect carrying capacity of ecosystems at different scales., HS-LS2-2. Use mathematical representations to support and revise explanations based on evidence about factors affecting biodiversity and populations in ecosystems of different scales.

    Initial exploration modified with permission from: “A light snack” 1997. Living in Water, National Aquarium in Baltimore, Kendall Hunt Publishing, Iowa.